Coding

Part:BBa_M1003:Design

Designed by: Alex Hatch   Group: UtahState_BE5930_-_S11   (2011-04-13)


HlyB membrane protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1286
    Illegal BglII site found at 1949
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 216
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for expression in E. coli while avoiding the BioBricks standard restriction sites.


Source

Genomic. Escherichia coli UT189

References